So Spade came out and started his first monologue joke, and Annie started to say something, and Spade said something like, "uuhhh right away you have something to say?" So she stops talking, and the other guest, who I can't remember who it was, says something and Spade laughed at it. So Annie was like, "oh he can talk, but I can't?" And Spade just kind of ignored that. So he continues with the monologue and she sits there with a hurt, sad look on her face and doesn't say a thing or laugh the whole time. I only notice this because I have been there - oversensitive about something, and once you are upset it is hard to just joke and forget it.
So, he notices she is upset and tries to tease her into laughing, and she does kind of come around but seems not herself most of the show. At one point during break you see him talking to her and throwing his cards down which could mean he was like calm down, or jokingly throwing them. Who knows.
I just don't think it is a good idea to get pissy with Spade, it's his show! I actually don't like her very much so if she isn't on the show again that would be ok with me.